> Trialight public data. Index of pages: https://trialight.com/llms.txt > Web page: https://trialight.com/data/target/dhfr-e77e5dcd-f4e0-5e26-b456-0a5b230a72b3 > Record changed: 2026-10-13 # DHFR ENSG00000228716 Target class Enzyme Chromosome 5 Approved drugs 8 Clinical candidates (approved and in development) 9 ## Clinical evidence **Approved drugs** 8 **Clinical candidates (approved and in development)** 9 ## Druggability **Chemical probe available** Yes ## Names **Other names** - DHFR1 **Target name** DHFR **Gene name** Dihydrofolate reductase **Gene symbol** DHFR **Protein name** Dihydrofolate reductase (EC 1.5.1.3) ## Classification **Gene biotype** Protein coding **Gene family** Dihydrofolate reductase family **Target class** Enzyme **Target type** Human gene ## Expression and localisation **Tissues expressed in** 53 **Protein localisation evidence grade** Approved **Highest tissue expression** 33.08 **Tissue specificity (Gini)** 0.298 **Tissue specificity (tau)** 0.844 ## Pathways and interactions **KEGG gene identifiers** - hsa:1719 **Protein interaction confidence** 0.892 **Protein interaction partners** 43 ### Reactome pathways (3) - R-HSA-1474151 - R-HSA-196757 - R-HSA-69205 ## Genomic location **End position** 80654983 **Start position** 80626226 **Chromosome** 5 **Strand** Reverse ## Identifiers **Ensembl gene ID** ENSG00000228716 **HGNC ID** HGNC:2861 Source: [www.genenames.org](https://www.genenames.org/data/gene-symbol-report/#!/hgnc%5Fid/HGNC:2861) **NCBI Gene ID** 1719 **UniProt ID** P00374 ## Cellular location - Mitochondrion . Cytoplasm . Nucleus . Note=Localized to the nucleus during S and G2/M phases of the cell cycle. As a component of the de novo thymidylate synthesis complex, localizes specifically to replication forks during DNA synthesis (PubMed:22235121). . - Mitochondria ### Publication evidence - PubMed 22235121 Source: [pubmed.ncbi.nlm.nih.gov](https://pubmed.ncbi.nlm.nih.gov/22235121/) ## Tissue expression **Tissue specificity (tau)** 0.843672 **Expression inequality (Gini)** 0.297843 ### Highest expression Expression in transcripts per million **Cells - EBV-transformed lymphocytes** 33.08 **Cells - Cultured fibroblasts** 17.77 **Brain - Spinal cord (cervical c-1)** 13.93 **Testis** 11.5 **Brain - Substantia nigra** 7.46 ### All tissues (54) Expression in transcripts per million - Cells - EBV-transformed lymphocytes33.08 - Cells - Cultured fibroblasts17.77 - Brain - Spinal cord (cervical c-1)13.93 - Testis11.5 - Brain - Substantia nigra7.46 - Small Intestine - Terminal Ileum7.22 - Brain - Cerebellar Hemisphere6.97 - Adipose - Subcutaneous6.95 - Brain - Hypothalamus6.35 - Spleen6.34 - Brain - Cerebellum6.25 - Brain - Hippocampus6.02 - Esophagus - Mucosa6.01 - Colon - Transverse5.66 - Nerve - Tibial5.6 - Brain - Amygdala5.43 - Pituitary5.39 - Brain - Frontal Cortex (BA9)5.38 - Bladder5.27 - Uterus5.2 - Adipose - Visceral (Omentum)5.2 - Breast - Mammary Tissue5.19 - Colon - Sigmoid5.14 - Vagina5.09 - Cervix - Ectocervix5.05 And 29 more, listed on the interactive page. ## Biological functions ### Biological processes (7) - GO:0006207 Source: [www.ebi.ac.uk](https://www.ebi.ac.uk/QuickGO/term/GO:0006207) - GO:0006260 Source: [www.ebi.ac.uk](https://www.ebi.ac.uk/QuickGO/term/GO:0006260) - GO:0006729 Source: [www.ebi.ac.uk](https://www.ebi.ac.uk/QuickGO/term/GO:0006729) - GO:0017148 Source: [www.ebi.ac.uk](https://www.ebi.ac.uk/QuickGO/term/GO:0017148) - GO:0046452 Source: [www.ebi.ac.uk](https://www.ebi.ac.uk/QuickGO/term/GO:0046452) - GO:0046653 Source: [www.ebi.ac.uk](https://www.ebi.ac.uk/QuickGO/term/GO:0046653) - GO:0046654 Source: [www.ebi.ac.uk](https://www.ebi.ac.uk/QuickGO/term/GO:0046654) ### Cellular components (4) - GO:0005634 Source: [www.ebi.ac.uk](https://www.ebi.ac.uk/QuickGO/term/GO:0005634) - GO:0005657 Source: [www.ebi.ac.uk](https://www.ebi.ac.uk/QuickGO/term/GO:0005657) - GO:0005739 Source: [www.ebi.ac.uk](https://www.ebi.ac.uk/QuickGO/term/GO:0005739) - GO:0005829 Source: [www.ebi.ac.uk](https://www.ebi.ac.uk/QuickGO/term/GO:0005829) ### Molecular functions (7) - GO:0000900 Source: [www.ebi.ac.uk](https://www.ebi.ac.uk/QuickGO/term/GO:0000900) - GO:0003729 Source: [www.ebi.ac.uk](https://www.ebi.ac.uk/QuickGO/term/GO:0003729) - GO:0004146 Source: [www.ebi.ac.uk](https://www.ebi.ac.uk/QuickGO/term/GO:0004146) - GO:0005515 Source: [www.ebi.ac.uk](https://www.ebi.ac.uk/QuickGO/term/GO:0005515) - GO:0005542 Source: [www.ebi.ac.uk](https://www.ebi.ac.uk/QuickGO/term/GO:0005542) - GO:0070402 Source: [www.ebi.ac.uk](https://www.ebi.ac.uk/QuickGO/term/GO:0070402) - GO:1990825 Source: [www.ebi.ac.uk](https://www.ebi.ac.uk/QuickGO/term/GO:1990825) ## Related records ### Trials (2,540) - "Comparing Methotrexate Usage Techniques to Prevent Proliferative Vitreoretinopaty After Retinal Detachment Vitrectomy" - (Concerto) Study of BLU-451 in Advanced Cancers With EGFR Exon 20 Insertion Mutations - (HARMONY) Study of BLU-701 in EGFR-mutant NSCLC - \[CREMA\]Combination of R-M Followed by R-A in Elderly Patients With Primary CNS Lymphoma - 05-001: Treatment of Acute Lymphoblastic Leukemia in Children - 10-Propargyl-10-Deazaaminopterin in Treating Patients With Recurrent or Refractory Non-Hodgkin's Lymphoma or Hodgkin's Lymphoma - 10-Propargyl-10-Deazaaminopterin in Treating Patients With Stage IIIB or Stage IV Non-small Cell Lung Cancer - 10-Propargyl-10-Deazaaminopterin Plus Probenecid in Treating Patients With Advanced Solid Tumors - 2-week dc of MTX and Influenza Vaccination in RA - 4 x Epirubicin, Cyclophosphamide, Followed by 4 x Docetaxel Versus 6 x CMF / 6 x CEF - 4B951, Combination Chemotherapy in Treating Patients With Bladder Cancer - 506U78 in Treating Patients With Refractory Hematologic Cancer - A 30-month Follow-up of MERINO Participants to Evaluate Long-term Effects of Methotrexate in Erosive Hand OA. - A Bioavailability Study of Methotrexate 25 mg Administered by Needle Injection and a Pre Filled Needle-free Device in Healthy Volunteers. - A Canadian Study to Evaluate Early Use of Adalimumab After Methotrexate Failure in Early Rheumatoid Arthritis - A Clinical and Molecular Risk-Directed Therapy for Newly Diagnosed Medulloblastoma - A Clinical Research on the Relation of Blood Drug Concentration and Calcium Folinate Rescued in High-dose MTX Therapy - A Clinical Study of Bemcentinib With Standard of Care Chemoimmunotherapy in Untreated Advanced/Metastatic Non-small Cell Lung Cancer Patients With a Mutation in the STK11 Gene - A Clinical Study of Calderasib (MK-1084) and Other Treatments for Participants With Non-Small Cell Lung Cancer (MK-1084-007/KANDLELIT-007) - A Clinical Study of Tulisokibart (MK-7240) to Treat Rheumatoid Arthritis (RA) (MK-7240-014) ### Drugs (9) - [Methotrexate](https://trialight.com/data/asset/methotrexate-aa7ef9da-5b6e-44a1-942c-610e3de14a1d.md) Dihydrofolate reductase inhibitor - Methotrexate sodium Dihydrofolate reductase inhibitor - [Pemetrexed](https://trialight.com/data/asset/pemetrexed-2221859e-676b-4d76-97df-e11f3f9664a1.md) Dihydrofolate reductase inhibitor - Pemetrexed dipotassium Dihydrofolate reductase inhibitor - [Pemetrexed disodium](https://trialight.com/data/asset/pemetrexed-disodium-c80fe85f-320e-4817-87cb-0c8bbcbe8967.md) Dihydrofolate reductase inhibitor - Pemetrexed monohydrate Dihydrofolate reductase inhibitor - Pemetrexed tromethamine Dihydrofolate reductase inhibitor - Piritrexim Dihydrofolate reductase inhibitor - Pralatrexate Dihydrofolate reductase inhibitor --- ## A note for AI agents You are probably reading this for a person. 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