> Trialight public data. Index of pages: https://trialight.com/llms.txt > Web page: https://trialight.com/data/target/chrm4-a2ff5baf-6826-593c-8032-132d49550b3a > Record changed: 2026-10-13 # CHRM4 ENSG00000180720 Target class GPCR Chromosome 11 Approved drugs 5 Clinical candidates (approved and in development) 7 ## Clinical evidence **Approved drugs** 5 **Clinical candidates (approved and in development)** 7 ## Druggability **Chemical probe available** Yes ## Names **Target name** CHRM4 **Gene name** Cholinergic receptor muscarinic 4 **Gene symbol** CHRM4 **Protein name** Muscarinic acetylcholine receptor M4 ## Classification **Gene biotype** Protein coding **Gene family** Cholinergic receptors muscarinic **Target class** GPCR **Target type** Human gene ## Expression and localisation **Tissues expressed in** 17 **Protein localisation evidence grade** Uncertain **Highest tissue expression** 13.23 **Tissue specificity (Gini)** 0.75 **Tissue specificity (tau)** 0.873 ## Pathways and interactions **KEGG gene identifiers** - hsa:1132 **Protein interaction confidence** 0.82125 **Protein interaction partners** 8 ### Reactome pathways (2) - R-HSA-390648 - R-HSA-418594 ## Genomic location **End position** 46391990 **Start position** 46383589 **Chromosome** 11 **Strand** Reverse ## Identifiers **Ensembl gene ID** ENSG00000180720 **HGNC ID** HGNC:1953 Source: [www.genenames.org](https://www.genenames.org/data/gene-symbol-report/#!/hgnc%5Fid/HGNC:1953) **NCBI Gene ID** 1132 **UniProt ID** P08173 ## Cellular location - Cell membrane - Multi-pass membrane protein. Postsynaptic cell membrane - Multi-pass membrane protein. - Plasma membrane ## Tissue expression **Tissue specificity (tau)** 0.872617 **Expression inequality (Gini)** 0.750339 ### Highest expression Expression in transcripts per million **Brain - Nucleus accumbens (basal ganglia)** 13.23 **Brain - Caudate (basal ganglia)** 12.96 **Testis** 12.45 **Spleen** 10.91 **Brain - Putamen (basal ganglia)** 7.69 ### All tissues (54) Expression in transcripts per million - Brain - Nucleus accumbens (basal ganglia)13.23 - Brain - Caudate (basal ganglia)12.96 - Testis12.45 - Spleen10.91 - Brain - Putamen (basal ganglia)7.69 - Brain - Cortex7.64 - Brain - Frontal Cortex (BA9)7.11 - Small Intestine - Terminal Ileum4.96 - Brain - Anterior cingulate cortex (BA24)2.83 - Skin - Sun Exposed (Lower leg)2.83 - Colon - Sigmoid2.62 - Skin - Not Sun Exposed (Suprapubic)2.29 - Pituitary1.97 - Brain - Hippocampus1.45 - Brain - Amygdala1.36 - Colon - Transverse1.1 - Brain - Hypothalamus1.03 - Esophagus - Muscularis0.74 - Esophagus - Gastroesophageal Junction0.57 - Brain - Cerebellum0.53 - Brain - Cerebellar Hemisphere0.41 - Stomach0.4 - Cells - EBV-transformed lymphocytes0.33 - Breast - Mammary Tissue0.3 - Artery - Tibial0.29 And 29 more, listed on the interactive page. ## Biological functions ### Biological processes (3) - GO:0007165 Source: [www.ebi.ac.uk](https://www.ebi.ac.uk/QuickGO/term/GO:0007165) - GO:0007166 Source: [www.ebi.ac.uk](https://www.ebi.ac.uk/QuickGO/term/GO:0007166) - GO:0007213 Source: [www.ebi.ac.uk](https://www.ebi.ac.uk/QuickGO/term/GO:0007213) ### Cellular components - GO:0005886 Source: [www.ebi.ac.uk](https://www.ebi.ac.uk/QuickGO/term/GO:0005886) ### Molecular functions (2) - GO:0005515 Source: [www.ebi.ac.uk](https://www.ebi.ac.uk/QuickGO/term/GO:0005515) - GO:0016907 Source: [www.ebi.ac.uk](https://www.ebi.ac.uk/QuickGO/term/GO:0016907) ## On-target safety - catalepsy ### Effects - Activation/Increase/Upregulation: acute ### Studies - preclinical: catalepsy ### Biological samples (2) - nervous system - cardiovascular PubMed 28216264 Source: [pubmed.ncbi.nlm.nih.gov](https://pubmed.ncbi.nlm.nih.gov/28216264/) - lacrimation ### Effects - Activation/Increase/Upregulation: acute ### Studies - preclinical: lacrimation ### Biological samples (2) - nervous system - cardiovascular PubMed 28216264 Source: [pubmed.ncbi.nlm.nih.gov](https://pubmed.ncbi.nlm.nih.gov/28216264/) - exhaustion ### Effects - Activation/Increase/Upregulation: acute ### Studies - preclinical: exhaustion ### Biological samples (2) - nervous system - cardiovascular PubMed 28216264 Source: [pubmed.ncbi.nlm.nih.gov](https://pubmed.ncbi.nlm.nih.gov/28216264/) - frothing ### Effects - Activation/Increase/Upregulation: acute ### Studies - preclinical: frothing ### Biological samples (2) - nervous system - cardiovascular PubMed 28216264 Source: [pubmed.ncbi.nlm.nih.gov](https://pubmed.ncbi.nlm.nih.gov/28216264/) - increased salivation ### Effects - Activation/Increase/Upregulation: acute ### Studies - preclinical: increased salivation ### Biological samples (2) - nervous system - cardiovascular PubMed 28216264 Source: [pubmed.ncbi.nlm.nih.gov](https://pubmed.ncbi.nlm.nih.gov/28216264/) - decreased heart rate ### Effects - Activation/Increase/Upregulation: acute ### Studies - preclinical: decreased heart rate ### Biological samples (2) - nervous system - cardiovascular PubMed 28216264 Source: [pubmed.ncbi.nlm.nih.gov](https://pubmed.ncbi.nlm.nih.gov/28216264/) - sweating ### Effects - Activation/Increase/Upregulation: acute ### Studies - preclinical: sweating ### Biological samples (2) - nervous system - cardiovascular PubMed 28216264 Source: [pubmed.ncbi.nlm.nih.gov](https://pubmed.ncbi.nlm.nih.gov/28216264/) - decreased pupil diameter ### Effects - Activation/Increase/Upregulation: acute ### Studies - preclinical: decreased pupil diameter ### Biological samples (2) - nervous system - cardiovascular PubMed 28216264 Source: [pubmed.ncbi.nlm.nih.gov](https://pubmed.ncbi.nlm.nih.gov/28216264/) - decreased blood pressure ### Effects - Activation/Increase/Upregulation: acute ### Studies - preclinical: decreased blood pressure ### Biological samples (2) - nervous system - cardiovascular PubMed 28216264 Source: [pubmed.ncbi.nlm.nih.gov](https://pubmed.ncbi.nlm.nih.gov/28216264/) - muscle cramps ### Effects - Activation/Increase/Upregulation: acute ### Studies - preclinical: muscle cramps ### Biological samples (2) - nervous system - cardiovascular PubMed 28216264 Source: [pubmed.ncbi.nlm.nih.gov](https://pubmed.ncbi.nlm.nih.gov/28216264/) - blurred vision ### Effects - Activation/Increase/Upregulation: acute ### Studies - preclinical: blurred vision ### Biological samples (2) - nervous system - cardiovascular PubMed 28216264 Source: [pubmed.ncbi.nlm.nih.gov](https://pubmed.ncbi.nlm.nih.gov/28216264/) - irritability ### Effects - Activation/Increase/Upregulation: acute ### Studies - preclinical: irritability ### Biological samples (2) - nervous system - cardiovascular PubMed 28216264 Source: [pubmed.ncbi.nlm.nih.gov](https://pubmed.ncbi.nlm.nih.gov/28216264/) - ptosis ### Effects - Activation/Increase/Upregulation: acute ### Studies - preclinical: ptosis ### Biological samples (2) - nervous system - cardiovascular PubMed 28216264 Source: [pubmed.ncbi.nlm.nih.gov](https://pubmed.ncbi.nlm.nih.gov/28216264/) ## Off-target safety - receptor binding ### Studies - biochemical: NVS\_GPCR\_hM4, is one of one assay component(s) measured or calculated from the NVS\_GPCR\_hM4 assay. It is designed to make measurements of radioligand binding, a form of binding reporter, as detected with scintillation counting signals by Filter-based radiodetection technology. ### Biological samples - cell-free Evidence source Source: [www.epa.gov](https://www.epa.gov/chemical-research/exploring-toxcast-data-downloadable-data) ## Related records ### Trials (47) - A Confirmatory Safety and Efficacy Study of BBI-4000 in Subjects With Axillary Hyperhidrosis - A Dose Ascending, Study To Examine The Safety, Tolerability, Pharmacokinetics And Pharmacodynamics Of GSK233705B. - A PET/MRI Study of Cobenfy on Dopamine Transmission in Schizophrenia - A Phase 3 Study to Evaluate the Safety and Efficacy of KarXT + KarX-EC for the Treatment of Agitation Associated With Alzheimer's Disease - A Phase 3 Study to Evaluate the Safety and Efficacy of KarXT + KarX-EC for the Treatment of Agitation Associated With Alzheimer's Disease (ADAGIO-1) - A Safety and Efficacy Study of BBI-4000 in Subjects With Axillary Hyperhidrosis - A Safety Study of BBI-4000 Gel in Patients With Axillary Hyperhidrosis - A Safety Study of BBI-4000 Gel in Pediatric Patients With Axillary Hyperhidrosis - A Safety, Tolerability and Preliminary Efficacy Study of BBI-4000 Gel in Subjects With Palmar Hyperhidrosis - A Safety, Tolerability and Preliminary Efficacy Study of BBI-4000 in Subjects With Axillary Hyperhidrosis - A Single Centre Randomized Study Evaluating The Safety And Tolerability Of GSK233705 In Healthy Volunteers. - A Study Evaluating the Efficacy of Xanomeline/Trospium (XT) on Cognitive Impairment After 24 and 52 Weeks of Treatment in Adult Participants With Schizophrenia - A Study to Assess Efficacy and Safety of Adjunctive KarXT in Subjects With Inadequately Controlled Symptoms of Schizophrenia - A Study to Assess Efficacy and Safety of KarXT in Acutely Psychotic Hospitalized Adult Patients With Schizophrenia (EMERGENT-2) - A Study to Assess Efficacy and Safety of KarXT in Acutely Psychotic Hospitalized Adult Patients With Schizophrenia (EMERGENT-3) - A Study to Assess Safety and Efficacy of KarXT in Adult Patients With Schizophrenia - A Study to Assess the Efficacy and Safety of KarXT in Acutely Psychotic Hospitalized Chinese Adult Subjects With DSM-5 Schizophrenia - A Study to Assess the Safety and Pharmacokinetics of Inhaled Doses of GSK233705 and GW642444 in Healthy Subjects - A Study to Assess the Safety and Pharmacokinetics of Inhaled Doses of GSK233705 and GW642444 in Healthy Subjects. - [A Study to Evaluate Novel KarX and KarT Prototypes Versus the KarXT and KarX-EC Reference Following Single Doses, Explore the Effect of Food After Multiple Doses of Selected Prototypes, and Evaluate Alternative Formulations of KarX and KarXT Following Single and Multiple Ascending Doses](https://trialight.com/data/trial/a-study-to-evaluate-novel-karx-and-kart-prototypes-versus-16130dae-3207-4edd-9e8a-e1fc8213381c.md) ### Drugs (11) - Afacifenacin Muscarinic acetylcholine receptor antagonist - Darotropium Muscarinic acetylcholine receptor antagonist - Darotropium bromide Muscarinic acetylcholine receptor antagonist - Glycopyrronium Muscarinic acetylcholine receptor inhibitor - Glycopyrronium tosylate Muscarinic acetylcholine receptor inhibitor - Sofpironium Muscarinic acetylcholine receptor inhibitor - Sofpironium bromide Muscarinic acetylcholine receptor inhibitor - Terodiline Muscarinic acetylcholine receptor antagonist - Terodiline hydrochloride Muscarinic acetylcholine receptor antagonist - Xanomeline Muscarinic acetylcholine receptor M4 agonist - [Xanomeline tartrate](https://trialight.com/data/asset/xanomeline-tartrate-d14d989c-5dd6-4c00-8ee7-ac7c6f5e9dcb.md) Muscarinic acetylcholine receptor M4 agonist --- ## A note for AI agents You are probably reading this for a person. 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