> Trialight public data. Index of pages: https://trialight.com/llms.txt
> Web page: https://trialight.com/data/asset/berahyaluronidase-alfa-1dda0ceb-c5c0-4aec-abf5-2bc9cdffc460
> Record changed: 2026-11-04
# Berahyaluronidase alfa
Highest phase reached
Phase 2
Source: [www.ebi.ac.uk](https://www.ebi.ac.uk/chembl/explore/compound/CHEMBL5314725)
Drug type
Enzyme
Source: [www.ebi.ac.uk](https://www.ebi.ac.uk/chembl/explore/compound/CHEMBL5314725)
## Development status
**Listing at source**
Listed
**Highest phase reached**
Phase 2
Source: [www.ebi.ac.uk](https://www.ebi.ac.uk/chembl/explore/compound/CHEMBL5314725)
## Names and chemistry
**Drug type**
Enzyme
Source: [www.ebi.ac.uk](https://www.ebi.ac.uk/chembl/explore/compound/CHEMBL5314725)
**Drug name**
Berahyaluronidase alfa
Source: [www.ebi.ac.uk](https://www.ebi.ac.uk/chembl/explore/compound/CHEMBL5314725)
### Other names (20)
- 3 305 hyaluronidase ph 20 human fusion protein with 303 322 hyaluronidase hyal 1 human fusion protein with 327 433 hyaluronidase ph 20 human
- 3-305-Hyaluronidase PH-20 (human) fusion protein with 303-322-hyaluronidase HYAL-1 (human) fusion protein with 327-433-hyaluronidase PH-20 (human)
- alt b4
- ALT-B4
- berahyaluronidasa alfa
- Berahyaluronidasa alfa
- berahyaluronidase alfa
- Berahyaluronidase alfa
- berahyaluronidase alfa \[INN\]
- BERAHYALURONIDASE ALFA \[USAN\]
- Berahyaluronidase alfa \[WHO-DD\]
- berahyaluronidase alfa inn
- berahyaluronidase alfa usan
- berahyaluronidase alfa who dd
- human hyaluronidase isozyme 5 hyal5 sperm adhesion molecule 1 spam1 hyaluronidase ph 20 ec 3 2 1 35 fragment 3 433 1 431 in the current sequence engineered variant with the structural domain replaced by the corresponding region of human hyaluronidase isozyme 1 hyal1 sup 306 sup tlsimrsmkscllddnymeti sup 326 sup sup 304 sup swentrtkescqaikeymdtt sup 324 sup produced in chinese hamster ovary cho cells cell line dg44 glycoform alfa
- human hyaluronidase isozyme-5 (HYAL5, sperm adhesion molecule 1, SPAM1, hyaluronidase PH-20, EC:3.2.1.35) fragment (3-433, 1-431 in the current sequence) engineered variant with the structural domain replaced by the corresponding region of human hyaluronidase isozyme-1 (HYAL1)(306TLSIMRSMKSCLLDDNYMETI326>304SWENTRTKESCQAIKEYMDTT324), produced in Chinese hamster ovary (CHO) cells, cell line DG44, glycoform alfa
- keytruda qlex component berahyaluronidase alfa
- KEYTRUDA QLEX COMPONENT Berahyaluronidase Alfa
- pembrolizumab and berahyaluronidase alfa
- pembrolizumab berahyaluronidase alfa
## Identifiers
**CAS registry numbers**
- 2636716-20-4
**ChEMBL ID**
CHEMBL5314725
Source: [www.ebi.ac.uk](https://www.ebi.ac.uk/chembl/explore/compound/CHEMBL5314725)
**RxNorm IDs (RxCUI)**
- 2723752
**FDA ingredient codes (UNII)**
- 9X4R4A4HQQ
## Regulatory information
### Other regulatory facts
**First in class**
No
**Orphan designation**
No
## Pharmacology
### Drug classification
**Pharmacology name**
pembrolizumab
**Type**
Antibody
**Drug name stem**
\-ase
### Open Targets indications (6)
- **Condition identifier**
EFO\_1001927
**Highest recorded phase**
2
- **Condition identifier**
MONDO\_0005086
**Highest recorded phase**
1
- **Condition identifier**
MONDO\_0005105
**Highest recorded phase**
1
- **Condition identifier**
MONDO\_0005233
**Highest recorded phase**
3
- **Condition identifier**
MONDO\_0008903
**Highest recorded phase**
3
- **Condition identifier**
MONDO\_0009348
**Highest recorded phase**
2
## Chemical properties
**Prodrug**
No
**Chirality**
Single enantiomer
**Inorganic**
No
**Natural product**
No
### Sequences (3)
- **Component**
Glycosylation sites (N)
**Sequence**
45 $ 129 $ 198 $ 217 $ 331 $ 356
- **Component**
Sequence
**Type**
Protein
**Sequence**
FRAPPVIPNVPFLWAWNAPSEFCLGKFDEPLDMSLFSFIGSPRINATGQGVTIFYVDRLGYYPYIDSITGVTVNGGIPQKISLQDHLDKAKKDITFYMPVDNLGMAVIDWEEWRPTWARNWKPKDVYKNRSIELVQQONVQLSLTEATEKAKQEFEKAGKDFLVETIKLGKLLRPNHLWGYYLFPDCYNHHYKKPGYNGSCFNVEIKRNDDLSWLWNESTALYPSIYLNTQQSPVAATLYVRNRVREAIRVSKIPDAKSPLPVFAYTRIVFTDQVLKFLSQDELVYTFGETVALGASGIVIWGSWENTRTKESCQAIKEYMDTTLNPYIINVTLAAKMCSQVLCQEQGVCIRKNWNSSDYLHLNPDNFAIQLEKGGKFTVRGKPTLEDLEQFSEKFYCSCYSTLSCKEKADVKDTDAVDVCIADGVCIDAF
- **Component**
Disulfide bridges
**Sequence**
23-314 $ 187-201 $ 339-350 $ 344-398 $ 400-406 $ 421-427
## National Drug Codes
- 0006-3083
- 0006-5083
## Related records
### Trials (14)
- A Clinical Study of Calderasib (MK-1084) and Other Treatments for Participants With Non-Small Cell Lung Cancer (MK-1084-007/KANDLELIT-007)
- A Clinical Study of Pembrolizumab (+) Berahyaluronidase Alfa (MK-3475A) to Treat Newly-diagnosed Metastatic Non-small Cell Lung Cancer (MK-3475A-F84)
- [A Clinical Trial of Adjuvant Intismeran (V940) With or Without Pembrolizumab Coformulated With Berahyaluronidase Alfa (MK-3475A) in High-Risk Stage I Non-Small Cell Lung Cancer (V940-014)](https://trialight.com/data/trial/a-clinical-trial-of-adjuvant-intismeran-v940-with-or-without-fbd7991a-01c0-4994-874e-a85283837202.md)
- A Clinical Trial of Sacituzumab Tirumotecan (Sac-TMT, MK-2870) and Pembrolizumab (MK-3475A) in Participants With Bladder Cancer (MK-2870-055)
- A Study of Participant Reported Preference for Subcutaneous Pembrolizumab Coformulated With Berahyaluronidase Alfa (MK-3475A) Over Intravenous Pembrolizumab (MK-3475) Formulation in Multiple Tumor Types (MK-3475A-F11)
- A Study of Pembrolizumab (+) Berahyaluronidase Alfa (MK-3475A) (Pembrolizumab Formulated With Berahyaluronidase Alfa (MK-5180)) in Japanese Participants With Recurrent or Metastatic Cutaneous Squamous Cell Carcinoma (R/M cSCC) or Locally Advanced (LA) Unresectable cSCC (MK-3475A-E39)
- A Study of Subcutaneous (SC) Pembrolizumab Coformulated With Berahyaluronidase Alfa (MK-3475A) vs Intravenous Pembrolizumab in Adult Participants With Metastatic Non-small Cell Lung Cancer (NSCLC) (MK-3475A-D77)
- A Study of Subcutaneous (SC) Pembrolizumab Coformulated With Berahyaluronidase Alfa (MK-3475A) vs Intravenous Pembrolizumab in Adult Participants With Metastatic Non-small Cell Lung Cancer (NSCLC) (MK-3475A-D77)-Japan Extension
- A Study to Evaluate the Bioavailability of Pembrolizumab (MK-3475) Via Subcutaneous (SC) Injection of Pembrolizumab Formulated With Berahyaluronidase Alfa (MK-5180) \[MK-3475A\] In Advanced Solid Tumors (MK-3475A-C18)
- A Study to Evaluate the Safety and Efficacy of Pembrolizumab (MK-3475) Coformulated With Berahyaluronidase Alfa (MK-3475A) in Participants With Relapsed or Refractory Classical Hodgkin Lymphoma (rrcHL) or Relapsed or Refractory Primary Mediastinal Large B-cell Lymphoma (rrPMBCL)(MK-3475A-F65)
- Effect of Tergase Injection on Reducing Edema After Bilateral Total Knee Arthroplasty
- Long-term Safety and Efficacy Extension Study for Participants With Advanced Tumors Who Are Currently on Treatment or in Follow-up in a Pembrolizumab (MK-3475) Study (MK-3475-587/KEYNOTE-587)
- Pharmacokinetics and Tolerability of Subcutaneous Dupilumab Coformulated With Berahyaluronidase Alfa and Dupilumab Without Berahyaluronidase Alfa in Healthy Participants
- Zanzalintinib Alone or in Combination With Pembrolizumab and Berahyaluronidase Alfa in Participants With Colorectal Cancer With Molecular Residual Disease
### In development for (5)
- Classic Hodgkin lymphoma Phase 2
- [Lung cancer](https://trialight.com/data/condition/lung-cancer-c4ca1fe0-9b3d-5957-a668-2d6cf26bd16a.md) Phase 3
- [Melanoma](https://trialight.com/data/condition/melanoma-457e9de6-dcd9-56fb-be48-0290fb4386ef.md) Phase 1
- [Non-small cell lung carcinoma](https://trialight.com/data/condition/non-small-cell-lung-carcinoma-a86c1d60-523f-5ea7-a5e1-39ed4a2296f8.md) Phase 3
- [Renal cell carcinoma](https://trialight.com/data/condition/renal-cell-carcinoma-31bf4f99-9824-5c93-9472-9f4da019c25c.md) Phase 1
---
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